Technician / Technicien(ne) - Mammalian Cell Culture & Immunoengineering

**Mission** ----------- The Molecular and Chemical Immunoengineering Group at EPFL develops molecular technologies to understand and engineer immune recognition and signaling. Our research combines protein engineering, chemical biology, molecular biology, and cell-based approaches to study T-cell recognition, post-translationally modified antigens, and synthetic immune-receptor signaling. To support our expanding experimental program, we are seeking a motivated and experienced laboratory technician with strong expertise in mammalian cell culture and cell-based assays. The successful candidate will work closely with postdoctoral researchers and students across several projects and will play an important role in establishing robust and reproducible cell-based experimental workflows in the laboratory. **Main duties and responsibilities** ------------------------------------ * **Mammalian cell culture:** Maintain and expand mammalian cell lines, including routine culture, freezing, thawing, cell banking, and quality control. * **Cell-based assays:** Perform routine signaling and functional assays, including stimulation experiments, dose-response and time-course assays, cell viability and proliferation measurements. * **Flow cytometry:** Perform sample preparation, staining, acquisition, and basic analysis for flow cytometry and phospho-flow experiments. * **Cell engineering:** Carry out transient transfection and lentiviral transduction and assist with the generation and maintenance of engineered stable cell lines. * **Primary immune-cell work:** Assist with isolation, culture, stimulation, and analysis of primary human immune cells, particularly T cells, when required. * **Molecular biology support:** Perform routine cloning, plasmid preparation, PCR, sequencing preparation, and related molecular biology procedures. * **Experimental support:** Work closely with researchers to establish standardized protocols and perform routine or repeated experiments supporting ongoing research projects. * **Laboratory organization:** Maintain cell stocks, reagents, inventories, and experimental records; assist with ordering and general laboratory organization. * **Safety and compliance:** Ensure adherence to EPFL biosafety guidelines and laboratory procedures and contribute to maintaining a safe and well-organized working environment. * **Equipment maintenance:** Assist with routine maintenance and coordination of servicing for relevant laboratory equipment. **Profile** ----------- **Required qualifications** * BSc, MSc, CFC/EFZ, or equivalent training in biology, biotechnology, bioengineering, immunology, or a related life science field. * At least two years of hands-on laboratory experience in an academic, clinical, or industrial research environment. * Strong practical experience with mammalian cell culture. * Experience with standard molecular and cell biology techniques. * Experience with flow cytometry. * Experience with viral transduction, transient