Post Doctoral Associate, Immunology, DNA damage, and Apoptosis

**Description** **Area**: Immunology, DNA damage, and Apoptosis **Duration**: 1 year with potential for extension pending availability of funding **Start Date**: Immediate **Salary**: $ CAD 60,000 The **Arnie Charbonneau Cancer Institute** in the **Cumming School of Medicine** at the University of Calgary is accepting applications for a **Postdoctoral Scholar in** **Immunology, DNA damage, and Apoptosis**. The laboratory of Dr. Holly Lee at the Arnie Charbonneau Cancer Institute, University of Calgary, is seeking a highly motivated Postdoctoral Associate with strong expertise in immunology, DNA damage, and apoptosis biology. The research program focuses on understanding how immune pressure shapes tumour evolution in multiple myeloma by altering apoptotic thresholds and tumour genomic mutability. The successful candidate will work with banked primary myeloma tumour and immune cell samples, human myeloma cell line models, and multi-omic datasets including scRNA-seq and whole-genome sequencing. Start date is flexible and the position will remain open until filled. The lab The H. Lee Lab is a new translational research group that leverages clinical tumour samples to investigate multiple myeloma tumour immunology, clonal evolution, and the development of novel immunotherapeutic strategies. The lab is embedded in the Arnie Charbonneau Cancer Institute and is a member of the Multiple Myeloma Research Group, which supports genomic and transcriptomic analysis. Our research platform includes: * Primary tumour sampling and immune cell assays * Genomic, transcriptomic, proteomic, and molecular profiling * Multiple myeloma human cell line models * Mouse models of immunotherapy * In vitro and in vivo evaluation of immunotherapeutic agents (T cell engagers, CAR T) * Tumour evolution and resistance modelling Terms and benefits The candidate will be enrolled in a comprehensive benefits program covering health, dental, life insurance, Accidental Death & Dismemberment, and disability. Two years of salary support is guaranteed, and the successful candidate will be supported in applying for external fellowships. **Job Description:** * Design and execute experiments involving gene knock-in and knock-out using molecular cloning, lentiviral production, and CRISPR-based editing * Perform functional assays with myeloma cell lines and primary immune cells, including T-cell engager coculture, apoptosis and DNA damage readouts, and flow cytometry * Analyze and interpret multi-omic data generated by the project, working with lab and core bioinformatics support * Contribute to study design and interpretation across the patient cohort and the model systems * Prepare manuscripts, abstracts, reviews, and scientific presentations * Co-mentor junior trainees, including a graduate student working on a related arm of the T-cell engager resistance project * Collaborate with core facilities (flow cytometry, genomics, animal facilities) * Maintain current knowledge of the