Researcher in Structural Biology: Mechanisms of Iron-Sulfur Cluster Assembly H/F

**Position description** ------------------------ ### **Category** Biology, biophysics and biochemistry ### **Contract** Fixed-term contract ### **Job title** Researcher in Structural Biology: Mechanisms of Iron-Sulfur Cluster Assembly H/F ### **Socio-professional category** Executive ### **Contract duration (months)** 24 ### **Job description** A research position with an initial term of two years is available to work on the structural characterization of protein complexes involved in the assembly and transport of iron-sulfur clusters to a client protein responsible for the biosynthesis of a key metabolic cofactor. This position is based in the Metalloproteins Unit at the Grenoble Institute of Structural Biology (IBS/IRIG), with a desired start date of March 1, 2027. The researcher fellow will carry out original research on the molecular mechanisms of an unusual iron-sulfur cluster assembly machinery that builds the cofactor directly within its client protein. Using a combination of structural biology, in vitro assays, and spectroscopic techniques, the candidate will capture and characterize the successive intermediates of this process. The work will rely mainly on single-particle cryo-electron microscopy and, where possible, X-ray crystallography, to resolve the molecular interactions involved and follow the stepwise assembly of the FeS cluster within the client protein. The long-term goal is to determine how generalizable this process is, in order to make better use of FeS enzymes in large-scale biotechnological applications. The researcher researcher will establish protocols to produce protein samples suitable for structural and spectroscopic analyses. They will also perform cryo-EM grid preparation, data processing, structural model refinement, and analysis. When possible, they will also prepare crystals and collect diffraction data. ### **Applicant Profile** The ideal candidate holds a Ph.D. and has a solid background in laboratory biochemistry, including protein expression and purification and a genuine interest in metalloproteins and enzymatic mechanisms. Experience working under anaerobic conditions is appreciated. Strong experience in structural biology, particularly in cryo-EM and/or X-ray crystallography, is an asset. Some affinity for computational tools, including an interest in basic programming for processing and analyzing structural data, will be greatly appreciated. We seek a motivated and open-minded researcher with strong communication skills, who thrives in a collaborative, multidisciplinary team. Prior knowledge of the French research system is not required, but would be a valuable asset for greater autonomy in the role. The successful candidate will join the Metalloproteins Unit at IBS and will work closely with Dr. Yvain Nicolet. The Metalloproteins Unit has a solid experience in the structural study of metalloproteins and has a set of 6 glove boxes to work “from gene to structure” in the absence of o